Two different sialidases, KDN-sialidase and regular sialidase in the starfish Asterina pectinifera

Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera.

Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28000. Optimum pH and temperature of A. pectinifera trypsin for hydro...

Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

Nuclear distribution of proliferating cell nuclear antigen (PCNA) in fertilized eggs of the starfish Asterina pectinifera.

Previous studies (Nomura et al. (1991) Dev. Biol. 143, 289-296 (1993) Dev. Biol. 159, 288-297) determined the time of DNA replication period (S phase) in starfish eggs fertilized either during or after oocyte maturation. Here proliferating cell nuclear antigen (PCNA) localized within nuclei of starfish eggs was detected with an anti-PCNA human antiserum. Using a confocal laser scanning microsco...

Nucleotide sequence and gene organization of the starfish Asterina pectinifera mitochondrial genome.

The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. The genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion, similar to those of other animal mtDNAs, with some of the genes overlapping each other. The gene organization is the same as that for another echinoderm, sea urchin, except for the inversion of a 4....

Establishment of embryonic axes in larvae of the starfish, Asterina pectinifera.

In order to clarify the relationships between the first cleavage plane and the embryonic axes, early cleavage pattern of the fertilized eggs of the starfish. Asterina pectinifera was reexamined. It was ascertained that the polar bodies were formed at the site to which the germinal vesicle had closely located before the initiation of the meiotic division, and that the first cleavage plane passed...